Composite

Part:BBa_K2737006:Design

Designed by: Xinyu Teng   Group: iGEM18_ECUST   (2018-10-06)


Plac-cecropin AD-T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 229
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence of cecropin AD is lack of initiation codon and termination codon.


Source

Coding sequence of cecropin AD is found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/AY328473 Combine Plac+RBS+ceropin AD+ T7 terminator.

References